Phenotypes and characteristics of human skin-derived precursors

doi:10.3969/j.issn.2095-4344.2013.36.018

Abstract

Abstract:

BACKGROUND: Human skin-derived precursors can be cultured for a long term in vitro, and differentiated into neurons, glial cells, smooth muscle cells, Schwann cells and cells with peripheral neurons phenotype.
OBJECTIVE: To investigate the culture conditions and multiple differentiation capacity of multipotential stem cells from human skin, especially the potentials of differentiating into neurons and osteoblasts.
METHODS: Human skin-derived precursor cells were cultured with trypsin digestion method, and identified with immunocytochemistry. Cells at passages 3-4 were induced to differentiate into neurons and osteoblasts, and underwent von Kossa staining protocol for calcium, chondrocyte induction, toluidine blue staining, immunohistochemical staining and Sudan black staining. The expression of nestin, vimentin, βIII-tubulin, S100 and collagen II in the human skin-derived precursors was detected.
RESULTS AND CONCLUSION: The human skin-derived precursor cells cultured with trypsin digestion method could proliferate and form suspending spheres, and nestin positive cells were detected at any time point of the culture. All the cultured cells expressed vimentin, and some adherent cells expressed βIII-tubulin. Human skin-derived precursor cells were induced with Salvia miltiorrhiza to differentiate into neuron-like cells, and expressed marker of nerve cells. Skin-derived precursors could be induced to differentiate into osteoblasts and von Kossa staining displayed black calcified nodules in the culture dish. Skin-derived precursors could also be induced to differentiate into chondrocytes, and toluidine blue staining was strongly positive, and some cells expressed collagen II, which suggested that, the differentiated cells contained chondrocytes. Experimental findings indicate that, skin contains multipotential stem cells that are capable of differentiating into osteoblasts, chondrocytes, Schwann cells and oligodendrocytes.

Key words: skin, multipotent stem cells, phenotype, neurons, osteoblasts, chondrocytes, Schwann cells, collagen type Ⅱ

CLC Number:

R394.2

Liu Gui-ying, Yang Li-ye, Li Wen-yu, Zheng Jia-kun. Phenotypes and characteristics of human skin-derived precursors[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.36.018 .

Figures/Tables 4

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